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Decline of genetic variability in a captive population of Pacific white shrimp Penaeus (Litopenaeus) vannamei using microsatellite and pedigree information Electron. J. Biotechnol.
Vela Avitúa,Sergio; Montaldo,Hugo H; Márquez Valdelamar,Laura; Campos Montes,Gabriel R; Castillo Juárez,Héctor.
Background: The objective of this study was to estimate the decline of genetic variability and the changes in effective population size in three shrimp populations. One was a wild population collected at several points in the Mexican Pacific Ocean. The other two populations were different generations (7 and 9) from a captive population selected for growth and survival. Microsatellite markers and pedigree were both used to assess genetic variability and effective population size. Results: Using 26 loci, both captive populations showed a decline in the expected heterozygosity (20%) and allelic diversity indices (48 to 91%) compared to the wild population (P < 0.05). The studied captive populations did not differ significantly from each other regarding...
Tipo: Journal article Palavras-chave: Effective population size; Genetic diversity; Heterozygosity; Microsatellites; Selection; Shrimp.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400009
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Fluorescent amplified fragment length polymorphism (fAFLP) analyses and genetic diversity in Litopenaeus vannamei (Penaeidae) Genet. Mol. Biol.
Gonçalves,Michelle Mantovani; Lemos,Manoel Victor Franco; Galetti Junior,Pedro Manoel; Freitas,Patrícia Domingues de; Furtado Neto,Manuel Antonio Andrade.
The Pacific white shrimp, Litopenaeus vannamei (Penaeidae), represents about 95% of all Brazilian shrimp production. The Brazilian L. vannamei foundation broodstock was made up of specimens collected from different American Pacific sites, but little information was collected on the genetic structure of the broodstock. We used the fluorescence amplified fragment length polymorphism (fAFLP) method to study the genetic diversity of L. vannamei broodstock lines 03CMF1 and 03CBF1 originally produced by breeder-shrimps imported mainly from Panama and Ecuador, although wild individuals from other localities may also have been used in producing these two lines. Our results showed a total of 93 polymorphic bands ranging from 50 to 500 bp, the mean Nei's genetic...
Tipo: Info:eu-repo/semantics/other Palavras-chave: FAFLP; Genetic diversity; Litopenaeus vannamei; Penaeidae; Shrimp.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200016
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Genetic distance between broodstocks of the marine shrimp Litopenaeus vannamei (Decapoda, Penaeidae) by mtDNA analyses Genet. Mol. Biol.
Francisco,Ana Karina de; Galetti Junior,Pedro Manoel.
In the constantly growing Brazilian shrimp industry the evaluation of genetic relationships between broodstocks is an useful tool for shrimp culture management programs. We established the genetic relationships between five broodstocks of the white marine shrimp Litopenaeus vannamei (Penaeidae) based on the sequencing of the mtDNA 16S rRNA and cytochrome oxidase I (COI) regions. Although no divergence was found between the broodstocks for the highly conservative 16S rRNA gene, we did find an 8.2% distance between L. vannamei and the Farfantepenaeus subtilis. Analyses of the COI region showed genetic distances of only 0.2 to 1% between the broodstocks, which contrasted with the 10.9% mean distance found between L. vannamei and F. subtilis. The small genetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shrimp; Litopenaeus vannamei; Genetic variation; MtDNA.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200014
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PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei Genet. Mol. Biol.
Freitas,Patrícia Domingues de; Galetti Junior,Pedro Manoel.
The genetic variation in two farmed strains (F3-Panama and F17-Venezuela) of the shrimp Litopenaeus vannamei was examined based on DNA multiloci analyses. Eighteen adults of each strain were analyzed by PCR using a set of VNTR core sequence primers. Genetic similarity, mean allele frequency, mean heterozygosity and the frequency of polymorphic loci were determined for both strains. A dendrogram of genetic similarity was produced by UPGMA clustering. The results for three primers (INS, M13, YN73) revealed different levels of genetic variation within the strains. The higher genetic similarity seen within strain F17 was apparently related to inbreeding, although a bottleneck effect could not be discarded. The low level of genetic variability of this strain...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Litopenaeus vannamei; Genetic diversity; Shrimp; VNTR; PCR.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400012
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Estágios de desenvolvimento gonadal de fêmeas do camarão-barba-ruça (Artemesia longinaris - Decapoda: Penaeidae) Iheringia, Sér. Zool.
Dumont,Luiz Felipe Cestari; D'Incao,Fernando.
The stages of gonadal development for the female of "barba-ruça" shrimp (Artemesia longinaris Bate, 1888) were characterized based on histological analysis. Four stages (immature, almost mature, ripe and spawned) were determined according to the structure and arrangement of cells in the ovary. Each stage corresponds macroscopically to a characteristic color, except stages I (immature) and IV (spawned), in which colors are very similar and can be distinguished only microscopically. The chromatic scale varies from white/translucent (stage I), neutral green (almost mature) to dark green (ripe). The mean size of cells was 56.9 µm (±3.5) (stage I), 127 µm (±2.6) (stage II) and 183 µm (±1.91) (stage III). The size frequency of cells was polimodal, and different...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artemesia; Shrimp; Reproduction; Gonadal histology.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212004000400006
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Manejo de compostos nitrogenados na carcinicultura. Infoteca-e
PEREIRA, A. M. L.; SILVA, A. E. M. da; OLIVEIRA, C. R. do R.; SILVA, D. A. da; OLIVEIRA, D. W. S; SANTOS, E. P. dos; GONÇALVES JUNIOR, G. F.; KATO, H. C. de A.; ABREU, J. L. de; SILVA, L. O. B. da; PIMENTEL, O. A. L. F.; LIMA, P. C. M. de; OLIVEIRA, V. Q. de; GÁLVEZ, A. O..
Introdução. Ciclo do nitrogênio na carcinicultura. Microrganismos responsáveis pela oxidação e transformação dos compostos nitrogenados. Estratégias de fertilização com carbono orgânico para o controle dos compostos nitrogenados no cultivo de camarão marinho. Estratégias de utilização do carbono inorgânico para auxiliar o controle dos compostos nitrogenados no cultivo de camarão marinho. Qualidade de água e sua importância para ajudar no controle dos compostos nitrogenados. Fertilização mineral em sistemas de baixa salinidade e sua influência sobre os compostos nitrogenados. Considerações finais. Referências.
Tipo: Livros Palavras-chave: Camarão marinho; BRS Aqua; Aquicultura; Cadeia Produtiva; Ciclo do Nitrogênio; Viveiro; Carcinicultura; Qualidade da Água; Aquaculture; Nitrogen cycle; Food chain; Nursery management; Shrimp; Nitrogen compounds; Litopenaeus vannamei; Water quality.
Ano: 2022 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1144229
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Mangrove increases resiliency of the French Guiana shrimp fishery facing global warming ArchiMer
Diop, Bassirou; Blanchard, Fabian; Sanz, Nicolas.
This paper deals with the biological, ecological and economic impact of global warming and mangrove habitat availability on the French Guiana shrimp fishery. A dynamic bio-economic model is built by employing a shrimp stock's growth function depending on Sea Surface Temperature (SST) and on mangrove surface. The model is empirically calibrated for the French Guiana shrimp fishery using time series collected over 1995–2011. First, two Cobb-Douglas functions, which describe shrimp's natural growth and harvest, are estimated. Then, a Maximum Economic Yield (MEY) harvest rule, based on the optimization of the net present value derived from fishing, is computed. Three management strategies are compared (Closure, Status Quo management, and MEY) under three...
Tipo: Text Palavras-chave: Renewable resources; Fishery bio-economic model; Global warming; Shrimp; Sea surface temperature; Mangrove.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00455/56672/58537.pdf
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Non-specific activation of antiviral immunity and induction of RNA interference may engage the same pathway in the Pacific white leg shrimp Litopenaeus vannamei ArchiMer
Labreuche, Yannick; Veloso, Artur; De La Vega, Enrique; Gross, Paul S.; Chapman, Robert W.; Browdy, Craig L.; Warr, Gregory W..
Many questions remain unanswered regarding RNAi-based mechanisms and dsRNA-induced antiviral immune responses in penaeid shrimp. In this study, we report the characterization in the white leg shrimp Litopenaeus vannamei of RNAi pathway associated proteins Lv-Ago 1 and Lv-Ago 2, two members of the Argonaute family of proteins, as well as Lv-sid 1, the first shrimp homologue of Sid-1, a membrane channel-forming protein implicated in the cellular import of dsRNA. To decipher their functional implication in RNAi-related phenomena, we monitored their relative expression following stimulation by specific and non-specific RNA duplexes of diverse length. The findings show that the length of small RNA duplexes plays a critical role in the activation of both...
Tipo: Text Palavras-chave: Shrimp; Litopenaeus vannamei; RNAi; DsRNA; Argonaute; Sid-1; Antiviral immunity.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00015/12652/9587.pdf
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"Summer Syndrome" in Litopenaeus stylirostris in New Caledonia: Pathology and epidemiology of the etiological agent, Vibrio nigripulchritudo ArchiMer
Goarant, Cyrille; Ansquer, Dominique; Herlin, Jose; Domalain, David; Imbert, Frederic; De Decker, Sophie.
The Summer Syndrome is a new shrimp disease that has been affecting a shrimp growout farm in New Caledonia since end of 1997. It was recognized to be caused by a systemic vibriosis due to Vibrio nigripulchritudo. This new disease turned out almost immediately enzootic in the shrimp farm involved and has affected all its crops ever since. Since the year 2000, V. nigripulchritudo strains have been found in several shrimp farms, although Summer Syndrome is still limited to one particular area, affecting, since 2003, two adjoining farms. As part of a multidisciplinary research program, a high-frequency survey was carried out during the summer 2002-2003 in two shrimp farms: one affected farm and another one in which pathogenic strains could be isolated but...
Tipo: Text Palavras-chave: Epidemiology; Vibrio; Diseases and their control; Shrimp.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1110.pdf
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Purification and characterization of infectious myonecrosis virus of penaeid shrimp ArchiMer
Poulos, Bonnie; Tang, K; Pantoja, C; Bonami, Jean-robert; Lightner, D.
The causative agent of myonecrosis affecting cultured Penaeus vannamei in Brazil was demonstrated to be a virus after purification of the agent from infected shrimp tissues. Purified viral particles were injected into specific pathogen-free P. vannamei, resulting in a disease that displayed the same characteristics as those found in the original shrimp used for purification. The virus was named infectious myonecrosis virus (IMNV). The viral particles were icosahedral in shape and 40 nm in diameter, with a buoyant density of 1(.)366 g ml(-1) in caesium chloride. The genome consisted of a single, double-stranded (dsRNA) molecule of 7560 bp. Sequencing of the viral genome revealed two non-overlapping open reading frames (ORFs). The 5' ORF (ORF 1, nt 136-4953)...
Tipo: Text Palavras-chave: Purification; Myonecrosis; Virus; Shrimp; Penaeid.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1504.pdf
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Identification de marqueurs génétiques de la virulence chez Vibrio nigripulchritudo, un pathogène de crevettes pénéides en Nouvelle-Calédonie ArchiMer
Reynaud, Yann.
Since 1997, a new pathology seasonally occurs in new caledonian shrimp farms during the warm season and was named Summer Syndrome. Diseased Litopenaeus stylirostris shrimp suffer from a septicemic vibriosis which was attributed to V. nigripulchritudo. Preliminary studies based on a collection of V. nigripulchritudo strains have brought to light different virulence levels according to experimental infections results; three virulence statuses were defined: highly (HP), moderately (MP) and non pathogenic (NP). The aim of this work was to genetically characterize virulent V. nigripulchritudo strains. In a first step the genetic diversity of 58 V. nigripulchritudo strains was analyzed by MLST and AP-PCR, revealing a cluster of HP and MP strains, characterized...
Tipo: Text Palavras-chave: Plasmide; SSH; Épidémiology; Virulence; Shrimp; Vibriosis; Vibrio nigripulchritudo; Plasmide; SSH; Épidémiologie; Virulence; Crevette; Vibriose; Vibrio nigripulchritudo.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-3906.pdf
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Digestive enzyme activity and food ingesta in juvenile shrimp Litopenaeus vannamei (Boone, 1931) as a function of body weight ArchiMer
Gamboa Delgado, Julián; Molina Poveda, César; Cahu, Chantal.
A study was conducted to evaluate variations of digestive enzyme activities in Litopenaeus vannamei (Boone) reared in commercial ponds under semi-intensive conditions. Shrimp were collected at each body weight increase of 2 g. As the shrimp grew (2-12 g), significant increases in the activities of lipase and chymotrypsin were observed. The total protease activity decreased from 6 g onwards. Trypsin activity showed a peak at 6 g and amylase activity increased two-fold after 2 g. Additionally, the stomach contents were analysed microscopically for shrimp between 2 and 10 g. Plant matter contributed above 30% of the total stomach content in 6-, 8- and 10-g shrimp. Detritus represented 58% and 62% of the total stomach content in 2- and 4-g shrimp,...
Tipo: Text Palavras-chave: Semi intensive culture; Stomach content; Digestive enzymes; Litopenaeus vannamei; Shrimp.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-902.pdf
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Nutrient and microbial dynamics in eutrophying shrimp ponds affected or unaffected by vibriosis ArchiMer
Lemonnier, Hugues; Courties, Claude; Mugnier, Chantal; Torreton, Jean-pascal; Herbland, Alain.
A field survey was conducted on two intensive shrimp farms using similar technical practices: one (DF) historically affected by a vibriosis, the other (HC) in which the pathogen has been observed although no mortality event has occurred. Because historical data suggest that eutrophication process may directly or indirectly play a role in the disease outbreak, we focussed our research on its dynamics. A higher variability of the phytoplanktonic compartment linked to an imbalance in the molar N:P ratio was observed in farm DF compared to farm HC, implying a modification on the linkage between the bacteria and phytoplankton compartments at DF. The beginning of the mortality outbreak at DF followed a shift from picoto nanophytoplankton. The organic matter...
Tipo: Text Palavras-chave: Aquaculture; Shrimp; Eutrophication; Ecosystem shift; Phytoplankton; Vibriosis.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00002/11368/8119.pdf
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Is It First the Egg or the Shrimp? – Diversity and Variation in Microbial Communities Colonizing Broods of the Vent Shrimp Rimicaris exoculata During Embryonic Development ArchiMer
Methou, Pierre; Hernández-ávila, Ivan; Aube, Johanne; Cueff-gauchard, Valerie; Gayet, Nicolas; Amand, Louis; Shillito, Bruce; Pradillon, Florence; Cambon-bonavita, Marie-anne.
Rimicaris exoculata is one of the most well-known and emblematic species of endemic vent fauna. Like many other species from these ecosystems, Rimicaris shrimps host important communities of chemosynthetic bacteria living in symbiosis with their host inside the cephalothorax and gut. For many of these symbiotic partners, the mode of transmission remains to be elucidated and the starting point of the symbiotic relationship is not yet defined, but could begin with the egg. In this study, we explored the proliferation of microbial communities on R. exoculata broods through embryonic development using a combination of NGS sequencing and microscopy approaches. Variations in abundance and diversity of egg microbial communities were analyzed in broods at...
Tipo: Text Palavras-chave: Hydrothermal; Shrimp; Microbial colonization; Alvinocarididae; Egg development.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00489/60067/63352.pdf
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Grow-out of sandfish Holothuria scabra in ponds shows that co-culture with shrimp Litopenaeus stylirostris is not viable ArchiMer
Bell, J; Agudo, N; Purcell, S; Blazer, P; Simutoga, M; Pham, Dominique; Della Patrona, Luc.
We examined the potential for producing the large numbers of sandfish (Holothuria scabra) needed for restocking programmes by co-culturing juveniles with the shrimp Litopenaeus stylirostris in earthen ponds. Our experiments in hapas within shrimp ponds were designed to detect any deleterious effects of sandfish on shrimp, and vice versa. These experiments showed that a high stocking density of juvenile sandfish had no significant effects on growth and survival of shrimp. However, survival and growth of sandfish reared with shrimp for 3 weeks were significantly lower than for sandfish reared alone. Increased stocking density of shrimp also had a significant negative effect on survival and/or growth of sandfish. A grow-out trial of juvenile sandfish in...
Tipo: Text Palavras-chave: Shrimp; Sea cucumbers; Sandfish; Pond culture; Predation; Co culture.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3590.pdf
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The role of microalgae in aquaculture: situation and trends ArchiMer
Muller-feuga, Arnaud.
Algae are utilized diversely in aquaculture, but their main applications are related to nutrition. They are used in toto, as a sole component or as a food additive to supply basic nutrients, color the flesh of salmonids or for other biological activities. The need for nutritional sources safer than traditional animal products has renewed interest in plants in general and algae in particular. This report deals principally with the nutritional role of microalgae in aquaculture.The larvae of molluscs, echinoderms and crustaceans as well as the live prey of some fish larvae feed on microalgae. Though attempts have been made to substitute inert particles for these micro-organisms which are difficult to produce, concentrate and store, only shrimp and live prey...
Tipo: Text Palavras-chave: Shrimp; Post larvae; Phytoplankton; Molluscs; Microalgae; Hatchery; Fish; Aquaculture.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-497.pdf
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Influence de l'environnement trophique de l'élevage en biofloc sur les performances physiologiques de la crevette Litopenaeus stylirostris : Étude de paramètres de la nutrition, de l'immunité et de la reproduction ArchiMer
Cardona, Emilie.
Biofloc is an intensive rearing system with zero or minimal water exchange where a diverse population of microorganisms (microalgae, zooplankton and bacteria) develops in association with organic matter to form the floc particles. These particles play the double role of biological filter and dietary supplement. This dissertation aims to better understand the process of this rearing system and its interactions with the Litopenaeus stylirostris shrimp. Two specific objectives were integrated within the framework of this general objective: (i) to measure the production gain from biofloc rearing and (ii) to study the interaction between biofloc environment and shrimp and to assess its role on production performances of shrimps. Thus, our results show...
Tipo: Text Palavras-chave: Biofloc; Crevette; Nutrition; Stress oxydant; Défenses antioxydantes; Immunité; Reproduction; Oeufs; Larves; Bactéries; Microbiote; Biofloc; Shrimp; Nutrition; Oxidative stress; Antioxidant defences; Immunity; Reproduction; Eggs; Larvae; Bacteria; Microbiota.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00272/38294/36492.pdf
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Arbitrarily primed PCR to type Vibrio spp. Pathogenic for shrimp ArchiMer
Goarant, Cyrille; Merien, Fabrice; Berthe, Franck; Mermoud, Isabelle; Perolat, Philippe.
A molecular typing study on Vibrio strains implicated in shrimp disease outbreaks in New Caledonia and Japan was conducted by using AP-PCR (arbitrarily primed PCR). It allowed rapid identification of isolates at the genospecies level and studies of infraspecific population structures of epidemiological interest. Clusters identified within the species Vibrio penaeicida were related to their area of origin, allowing discrimination between Japanese and New Caledonian isolates, as well as between those from two different bays in New Caledonia separated by only 50 km. Other subclusters of New Caledonian V. penaeicida isolates could be identified, but it was not possible to link those differences to accurate epidemiological features. This contribution of AP-PCR...
Tipo: Text Palavras-chave: Vibrio spp.; Shrimp; Molecular typing.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-2734.pdf
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Selection and characterization of potential probiotic bacteria for Litopenaeus stylirostris shrimp hatcheries in New Caledonia ArchiMer
Pham, Dominique; Ansquer, Dominique; Chevalier, Anne; Dauga, Clement; Peyramale, Aude; Wabete, Nelly; Labreuche, Yannick.
In New Caledonia, shrimp hatcheries are confronted with mass mortality in the larval stages, a phenomenon poorly understood as no specific causative agent has been identified. This has resulted in an excessive use of prophylactic antibiotics, although their adverse effects in aquaculture are notorious. The present work was thus aimed at selecting potential probiotic strains for penaeid hatcheries. From a pool of more than 400 marine bacterial isolates sampled from the local marine environment seven strains exhibited in vitro antagonistic activity towards Vibrio harveyi. These isolates were characterized both phenotypically and genotypically using a biochemical approach and 16S rDNA sequencing. Six out of these seven strains were found to belong to the...
Tipo: Text Palavras-chave: Shrimp; Litopenaeus stylirostris; Probiotic; Hatchery; Pseudoalteromonas; Immune response.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00190/30129/28591.pdf
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Bacterial community characterization of water and intestine of the shrimp Litopenaeus stylirostris in a biofloc system ArchiMer
Cardona, Emilie; Gueguen, Yannick; Magré, Kevin; Lorgeoux, Bénédicte; Piquemal, David; Pierrat, Fabien; Noguier, Florian; Saulnier, Denis.
Background Biofloc technology (BFT), a rearing method with little or no water exchange, is gaining popularity in aquaculture. In the water column, such systems develop conglomerates of microbes, algae and protozoa, together with detritus and dead organic particles. The intensive microbial community presents in these systems can be used as a pond water quality treatment system, and the microbial protein can serve as a feed additive. The current problem with BFT is the difficulty of controlling its bacterial community composition for both optimal water quality and optimal shrimp health. The main objective of the present study was to investigate microbial diversity of samples obtained from different culture environments (Biofloc technology and clear...
Tipo: Text Palavras-chave: Shrimp; Biofloc; Clear sea water; Bacterial communities; Culture environment; Microbiota.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00346/45694/45314.pdf
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